TABLE 2.
HP1 tethering antagonizes VP16-induced chromatin decondensation in a Dicer-independent manner
| Plasmid | No. of cells showing chromatin morphology and peroxisomal CFP signals (n = 50)
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Control siRNA-transfected cells
|
Dicer siRNA-transfected cells
|
|||||||
| Opena
|
Closedb
|
Open
|
Closed
|
|||||
| Per+c | Per− | Per+ | Per− | Per+ | Per− | Per+ | Per− | |
| VP16+pBlued | 45 | 0 | 3 | 2 | 44 | 3 | 2 | 1 |
| VP16+ΔVP16 | 36 | 3 | 9 | 2 | 34 | 3 | 6 | 7 |
| VP16+HP1α | 4 | 12 | 3 | 31 | 4 | 9 | 1 | 36 |
| VP16+HP1β | 1 | 15 | 1 | 33 | 2 | 6 | 3 | 39 |
| VP16+HP1γ | 3 | 11 | 2 | 34 | 6 | 8 | 0 | 36 |
a
Open, heterogeneous and extended locus.
b
Closed, homogeneous and compact locus.
c
Per, peroxisomal CFP signals.
d
pBlue, pBluescriptII KS(−).