Figure 3.

Nuclear phenotypes and cell cycle progression of tub1 mutants. Large-budded cells were examined both by ascertaining their frequency in a growing population of cells and by staining their nuclei with DAPI. Cells were examined both at 25°C and after a shift to 11°C for two generations. In A, the percentage of large-budded cells with an undivided nucleus was determined; in B, the percentage of large-budded cells with a nucleus away from its normal position near the bud neck was determined; and in C, the percentage of large-budded cells (bud diameter greater than one-half of the mother cell's body) in the population was determined.