Table 2.
Yeast strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| YPH102 | MAT∝ ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 | Sikorski and Hieter, 1989 |
| YPH250 | MATa ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 trp1-Δ1 | Sikorski and Hieter, 1989 |
| DBY786 | MAT∝ trp1-1 | This laboratory |
| DBY789 | MATa trp1-1 | This laboratory |
| KRY73 | MAT∝ ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 | This study |
| plasmid: pRB326 | ||
| DBY6590 | MAT∝ ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 tub1Δ::HIS3 | This study |
| plasmid: pRB326 | ||
| DBY6591 | MATa ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 trp1-Δ1 TUB1-LYS2 | This study |
| KRY76 (DBY6590 × 6591) | MATa/∝ ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 trp1-Δ1/TRP1 TUB1-LYS2/tub1Δ::HIS3 | This study |
| plasmid: pRB326 | ||
| DBY6592 | MAT∝ lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 TUB1-LYS2 | This study |
| plasmid: pRB326 | ||
| DBY6593 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1Δ::HIS3 | This study |
| plasmid: pRB326 | ||
| DBY6596 | MATa/∝ lys2-801/lys2-801 ade2-101/ade2-101 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB1-LYS2/tub1Δ::HIS3 | This study |
| plasmid: pRB326 | ||
| DBY6598 | MAT∝ lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 TUB1-LYS2 tub3Δ::HIS3 | This study |
| plasmid: pRB326 | ||
| DBY6599 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 TUB1-LYS2 tub3Δ::HIS3 | This study |
| plasmid: pRB326 | ||
| DBY6600 | MATa/∝ lys2-801/lys2-801 ade2-101/ade2-101 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB1-LYS2/TUB1-LEU2 | This study |
| plasmid pRB326 | ||
| DBY6601–6653 | Like DBY6600, but with tub1 Ala-scan alleles, -801 to -853, respectively | This study |
| DBY6654 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 TUB1–LEU2 | This study |
| DBY6655 | MAT∝ lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 TUB1–LEU2 | This study |
| DBY6656–6761 | Like DBY6654 and 6655, but with tub1 Ala-scan alleles, -801 to -853, respectively (2 each, 1 of each mating type) | This study |
| DBY6762 | MATa/∝ lys2-801/lys2-801 ade2-101/ade2-101 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB1-LYS2/TUB1-LEU2 tub3Δ::HIS3/TUB3 | This study |
| plasmid: pRB326 | ||
| DBY6816 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 TUB1–LEU2 tub3Δ::HIS3 | This study |
| DBY6822 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-803–LEU2 tub3Δ::HIS3 | This study |
| DBY6824 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-804–LEU2 tub3Δ::HIS3 | This study |
| DBY6826 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-805–LEU2 tub3Δ::HIS3 | This study |
| DBY6828 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-806–LEU2 tub3Δ::HIS3 | This study |
| DBY6830 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-807–LEU2 tub3Δ::HIS3 | This study |
| DBY6838 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-811–LEU2 tub3Δ::HIS3 | This study |
| DBY6840 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-812–LEU2 tub3Δ::HIS3 | This study |
| DBY6846 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-815–LEU2 tub3Δ::HIS3 | This study |
| DBY6854 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-819–LEU2 tub3Δ::HIS3 | This study |
| DBY6866 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-825–LEU2 tub3Δ::HIS3 | This study |
| DBY6868 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-826–LEU2 tub3Δ::HIS3 | This study |
| DBY6878 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-831–LEU2 tub3Δ::HIS3 | This study |
| DBY6880 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-832–LEU2 tub3Δ::HIS3 | This study |
| DBY6882 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-833–LEU2 tub3Δ::HIS3 | This study |
| DBY6886 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-835–LEU2 tub3Δ::HIS3 | This study |
| DBY6894 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-839–LEU2 tub3Δ::HIS3 | This study |
| DBY6898 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-841–LEU2 tub3Δ::HIS3 | This study |
| DBY6902 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-843–LEU2 tub3Δ::HIS3 | This study |
| DBY6916 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-850–LEU2 tub3Δ::HIS3 | This study |
| DBY6918 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-851–LEU2 tub3Δ::HIS3 | This study |
| DBY6920 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 tub1-852–LEU2 tub3Δ::HIS3 | This study |
| DBY7830 | MAT∝ lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 TUB1-LYS2 tub2-201 | Schwartz et al., 1997 |
| plasmid: pRB326 | ||
| DBY7051 | MATa ACT1::HIS his3-Δ200 leu2-3,112 ura3-52 ade4 tub2-201 | This laboratory |
| DBY8154 | MATa lys2-801 ade2-101 his3-Δ200 leu2-Δ1 ura3-52 TUB1-LEU2 tub2-201 | This study |