Table 3.

Summary of mutations

tub1 allele	Amino acid substitutions	DNA sequence changesa	Diagnostic restriction sites	Phenotypeb
801	R2A, E3A	GCTGCA	Gain PstI	Cs, Ts, BenSS
		AGAGAA
802	E27A, H28A	GCAGCC	Gain BbvI	RL
		GAGCAC
803	K31A, D33A	GCGCCGGCT	Gain HinP1I,	BenSS
		AAGCCGGAT	Lose BsaBI
804	H35A, E37A, D38A	GCTCTAGCAGCT	Gain PvuII,	BenSS
		CATCTAGAAGAT	Lose BsaBI
805	K42A, K44A	GCGCCGGCG	Gain HinP1I	BenSS
		AAGCCGAAG
806	E47A, E48A	GCAGCT	Gain PvuII	BenR
		GAAGAG
807	H55A, E56A	GCTGCA	Gain BbvI	BenSS
		CATGAA
808	K61A, R65A	GCGTTCGTTCCAGCT	Gain PvuII	RL
		AAGTTCGTTCCAAGG
809	D70A, E72A	GCTTTAGCG	Gain HinP1I	Slow growth,
		GATTTAGAG		BenSS
810	D77A, E78A, R80A	GCAGCAGTCGCT	Gain BbvI	BenSS
		GACGAAGTCCGT
811	K85A, D86A	GCTGCC	Gain BbvI	WT
		AAGGAC
812	H89A, E91A	GCTCCAGCA	Gain BpmI	BenSS
		CATCCAGAA
813	K97A, E98A, D99A	GCGGCGGCC	Gain NotI	Slow growth,
		AAGGAGGAC		BenSS
814	R106A, H108A	GCTGGCGCT	Gain PvuII	Slow growth,
		AGAGGCCAT		BenSS
815	R113A, E114A	GCAGCA	Gain BbvI	WT
		AGAGAA
816	D118A, D121A, R122A	GCTGTTCTGGCTGCG	Gain BbvI	Cs, BenSS
		GATGTTCTGGATAGG
817	R124A, K125A	GCAGCA	Gain BbvI	BenSS
		AGAAAA
818	D128A, D131A	GCCCAATGTGCA	Gain BsgI	Cs, BenSS
		GACCAATGTGGAT
819	T146A	GCA	Gain BsgI,	BenSS
		ACT	BspMI
820	E156A, E157A	GCAGCA	Gain BbvI	DL
		GAAGAA
821	E161A, K164A, K165A	GCATACGGTGCGGCA	Gain Fnu4HI	BenSS
		GAATACGGTAAGAAA
822	K167A, E169A	GCGCTGGCA	Gain HinP1I	Slow growth,
		AAGCTGGAA		BenSS
823	E197A, H198A, D200A	GCAGCTGCAGCT	Gain PstI, PvuII	RL
		GAACATGCAGAT
824	D206A, E208A	GCGAATGCG	Gain NruI,	Cs, BenSS
		GATAATGAG	BsmI
825	D212A, K215A, R216A	GCCATGTGCGCAGCA	Gain BbvI	BenR
		GACATGTGCAAAAGA
826	D219A, R222A	GCTATCCCAGCA	Lose EcoRV	BenR
		GATATCCCAAGA
827	R244A, D246A	GCATTCGCG	Gain BstUI	Slow growth,
		AGATTCGAG		BenSS
828	D252A, E255A	GCTTTGAACGCG	Gain MluI	DL
		GATTTGAACGAA
829	R265A, H267A	GCAATTGCT	Gain MunI,	Slow growth,
		AGAATTCAT	Lose EcoRI	BenSS
830	K279A, K281A	GCATCAGCT	Gain PvuII	BenSS
		AAATCAAAG
831	H284A, E285A	GCTGCG	Gain Fnu4HI,	Cs, BenSS
		CATGAG	Lose HinfI, PleI
832	K305A, D307A	GCGTGTGCA	Gain ApaLI	BenR
		AAGTGTGAT
833	R309A, D310A, K312A	GCAGCTGGTGCA	Gain PvuII,	BenSS
		AGAGATGGTAAA	Fnu4HI
834	R321A, D323A	GCGGGTGCT	Gain BstUI	Slow growth,
		AGGGGTGAT		BenSS
835	R327A, D328A,	GCTGTTCAAGCA	Gain PvuII	WT
	R331A	GATGTTCAAAGA
836	E334A, K337A	GCGCAGGTGGCA	Gain BstUI,	Cs, Ts, BenSS
		GAGCAGGTGAAA	HinP1I
837	K339A, K340A	GCGGCG	Gain Fnu4HI,	BenSS
		AAGAAG	BstUI, Lose  BbsI
838	D373A, R374A	GCTGCA	Gain PstI, SfcI	BenSS
		GATAGG
839	E387A, K390A, R391A	GCAGCTTGGGCGGCA	Gain PstI	BenR
		GAGGCTTGGAAGAGA
840	D393A, R394A, K395A, D397A	GCTGCAGCATTCGCT	Gain PstI,	RL
		GATAGAAAATTCGAT	BsmI, SfcI,  Lose ClaI
841	D393A, R394A	GCTGCA	Gain PstI	BenR
		GATAGA
842	K395A, D397A	GCATTCGCT	Gain BsmI	Cs, Ts, BenSS
		AAATTCGAT
843	K395A	GCA	Gain BsmI	WT
		AAA
844	D397A	GCA	Gain AseI	BenSS
		GAT
845	K402A, R403A	GCAGCT	Gain PvuII	Slow growth, BenSS
		AAACGT		BenSS
846	E412A, E415A	GCAGGTATGGCA	Gain BsgI,	Cs, Ts, BenSS
		GAAGGTATGGAA	BspMI
847	E416A, E418A	GCAGGTGCA	Gain BsmI,	Slow growth,
		GAAGGTGAA	BspMI, Lose  EcoRI	BenSS
848	E421A, R423A,	GCAGCTGCAGCAGCT	Gain PstI, PvuII	Slow growth,
	E424A, D425A	GAAGCTAGAGAAGAT		BenSS
849	E430A, R431A, D432A	GCTGCAGCT	Gain PstI	Slow growth,
		GAAAGAGAT		BenSS
850	E435A, D439A	GCGGTGGGTGCCGCC	Gain BstUI	BenSS
		GAAGTGGGTGCCGAC
851	E443A, E444A, E445A,	GCTGCAGCGGCA	Gain PstI	BenSS
	E446A	GAGGAAGAGGAA
852	F447A	GCT	Gain HindIII	BenSS
		TTT
853	E184A	GCG	Gain HinP1I	Slow growth,
		GAG		BenSS

^aThe mutant sequence is above the corresponding wild-type sequence. Altered nucleotides are in bold; altered codons are underlined. 

^bBenR, benomyl resistant; BenSS, benomyl supersensitive; Cs, cold-sensitive; DL, dominant lethal; RL, recessive lethal; Slow growth, impaired growth at all temperatures; Ts, heat-sensitive; WT, wild-type.