Table 4.

tub1 and tub1 tub3 mutant phenotypes

Category of benomyl sensitivitya	tub1 allele	tub1 phenotypeb	tub1 tub3Δ phenotypeb
1	801	BenSS, Cs, Ts	SL
	809	BenSS, slow	SL
	813	BenSS, slow	SL
	814	BenSS, slow	SL
	817	BenSS	SL
	822	BenSS, slow	SL
	824	BenSS, Cs	SL
	827	BenSS, slow	SL
	829	BenSS, slow	SL
	833	BenSS	BenSS, slow
	834	BenSS, slow	SL
	836	BenSS, Cs, Ts	SL
2	810	BenSS	SL
	816	BenSS, Cs	SL
	818	BenSS, Cs	SL
	819	BenSS	BenSS, slow
	830	BenSS	SL
	837	BenSS	SL
	845	BenSS, slow	SL
	846	BenSS, Cs, Ts	SL
	849	BenSS, slow	SL
	853	BenSS, slow	SL
3	803	BenSS	BenSS, slow
	804	BenSS	BenSS, Cs
	805	BenSS	BenSS, slow
	807	BenSS	BenSS, slow
	812	BenSS	BenSS, Ts
	821	BenSS	SL
	831	BenSS, Cs	BenSS, slow
	838	BenSS	SL
	842	BenSS, Cs, Ts	SL
	844	BenSS, Cs, Ts	SL
	847	BenSS, slow	SL
	848	BenSS, slow	SL
	850	BenSS	BenSS, slow
	851	BenSS	BenSS, slow
	852	BenSS	BenSS
4	TUB1	WT	BenSS
	811	WT	BenSS
	815	WT	BenSS
	835	WT	BenSS
	843	WT	BenSS, Cs
5	806	BenR	BenSS
	825	BenR	BenSS
	826	BenR	BenSS
	832	BenR	BenSS
	839	BenR	BenSS
	841	BenR	BenSS, slow

^a1, more sensitive than tub3Δ; 2, sensitivity equal to tub3Δ; 3, less sensitive than tub3Δ but more sensitive than wild type; 4, sensitivity equal to wild type; 5, less sensitive than wild type (benomyl-resistant). 

^bBenR, benomyl resistant; BenSS, benomyl supersensitive; Cs, cold-sensitive; SL, synthetic lethal; slow, slow growth at all temperatures; Ts, heat-sensitive; WT, wild-type.