Table 5.
Microtubule phenotypes of tub1 mutants and tub1 tub3Δ double mutants
| tub1 mutant | Microtubule class | Growth phenotype | tub1 tub3Δ mutant | Microtubule class | Growth phenotype |
|---|---|---|---|---|---|
| 800 (wild-type) | 3 | WT | 800 tub3Δ | 3 | BenSS |
| 801 | 3 | BenSS, Cs, Ts | 803 tub3Δ | 1 | BenSS, slow |
| 806 | 3 | BenR | 805 tub3Δ | 3 | BenSS, slow |
| 809 | 1 | BenSS, slow | 806 tub3Δ | 3 | BenSS |
| 813 | 3 | BenSS, slow | 807 tub3Δ | 1 | BenSS, slow |
| 814 | 3 | BenSS, slow | 812 tub3Δ | 3 | BenSS, Ts |
| 822 | 3 | BenSS, slow | 819 tub3Δ | 3 | BenSS, slow |
| 827 | 1 | BenSS, slow | 825 tub3Δ | 3 | BenSS |
| 829 | 3 | BenSS, slow | 831 tub3Δ | 3 | BenSS, slow |
| 831 | 2 | BenSS, Cs | 833 tub3Δ | 3 | BenSS, slow |
| 834 | 1 | BenSS, slow | 850 tub3Δ | 3 | BenSS, slow |
| 845 | 3 | BenSS, slow | 851 tub3Δ | 1 | BenSS, slow |
| 846 | 3 | BenSS, Cs, Ts | 852 tub3Δ | 3 | BenSS |
| 847 | 3 | BenSS, slow | |||
| 848 | 3 | BenSS, slow | |||
| 849 | 3 | BenSS, slow | |||
| 853 | 3 | BenSS, slow |
Microtubules from mutant cells grown for two generations at 11°C were visualized using indirect immunofluorescence. Intensity was scored in comparison with wild type (TUB1-800), which was included in every experiment. Mutants are divided into three categories according to their microtubule phenotypes: 1) very diminished or no microtubules, 2) excess microtubules, and 3) normal microtubules, sometimes fainter than usual. Growth phenotypes are shown for comparison, and abbreviations are as listed in Table 4.