TABLE 1.
Yeast strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| BY4741 | MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | ATCC |
| orfΔ | BY4741 orfΔ::kanMX4 | Open Biosystems |
| FR1025 | BY4741 doa1Δ::LEU2 | This study |
| FR381 | BY4741 ubi4Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR684 | BY4741 ubx5Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR682 | BY4741 ubx6Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR683 | BY4741 ubx7Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR432 | BY4741 shp1Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR561 | BY4741 ubp7Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR550 | BY4741 rad6Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR552 | BY4741 rad5Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR553 | BY4741 rad18Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR1021 | BY4741 mms2Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR1024 | BY4741 ubc13Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR376 | BY4741 srs2Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR663 | BY4741 bre1Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR1026 | BY4741 lge1Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR685 | BY4741 cdc73Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR664 | BY4741 ubp8Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR686 | BY4741 htb2Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR687 | BY4741 hda1Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR688 | BY4741 htz1Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR555 | BY4741 rad9Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR1218 | BY4741 ubp4Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR1223 | BY4741 rad12Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR1225 | BY4741 rad14Δ::kanMX4 doa1Δ::LEU2 | This study |
| FR521 | BY4741 DOA1-13myc | This study |
| MHY501 | MATalys2-801 leu2-3,112 ura3-52 his3-Δ200 trp1-1 gal2 | M. Hochstrasser |
| MHY1227 | MATalys2-801 leu2-3,112 ura3-52 his3-Δ200 trp1-1 ubp10::HIS3 | M. Hochstrasser |
| FR1231 | MHY501 MATa | This study |
| FR1229 | MHY501 MATadoa1Δ::LEU2 | This study |
| FR1232 | MHY501 MATaubp10Δ::HIS3 | This study |
| FR1230 | MHY501 MATaubp10Δ::HIS3 doa1Δ::LEU2 | This study |
| W1588-4C | MATaade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | R. Rothstein |
| FR657 | W1588-4C doa1Δ::LEU2 | This study |
| SUB280 | MATalys2-801 leu2-3,112 ura3-52 his3-Δ200 trp1-1 [am] ubi1-Δ1::TRP1 ubi2-Δ2::ura3 ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39, pUB100) | D. Finley |
| FR512 | SUB280 doa1Δ::kanMX6 | This study |
| SUB413 | MATa lys2-801 leu2-3,112 ura3-52 his3-Δ200 trp1-1 [am] ubi1-Δ1::TRP1 ubi2-Δ2::ura3 ubi3-Δub-2 ubi4-Δ2::LEU2 [Ub K63R] [pUB100] | D. Finley |
| FR514 | SUB413 doa1Δ::kanMX6 | This study |
| Y0003 | MATa his3-Δ200 leu2-3,112 lys2-801 trp1-1 [am] ura3-52 | S. Jentsch |
| FR1023 | Y0003 doa1Δ::LEU2 | This study |
| FR1088 | Y0003 POL30-3HA | This study |
| FR1090 | Y0003 POL30-3HA doa1Δ::LEU2 | This study |
| Y1192 | Y0003 pol30-K164R | S. Jentsch |
| FR1022 | Y1192 doa1Δ::LEU2 | This study |
| FR1105 | Y1192 pol30-3HA | This study |
| DOA1-TAP | BY4741 DOA1-TAP | Open Biosystems |
| EGY48 | MATaura3 trp l his3 6lexAop-LEU2 | Origene |