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. 2006 Jun;26(11):4240–4256. doi: 10.1128/MCB.02124-05

FIG. 1.

FIG. 1.

Cyclin D1-deficient cells have increased stress fiber formation. (A) cyclin D1+/+ and cyclin D1−/− MEFs were plated on six-well plates and examined by phase-contrast microscopy (×10). Scale bar, 50 μm. (B) Cellular diameter assessed by Multisizer 3 Coulter counter. (C) Rhodamine-phalloidin staining of cyclin D1+/+ or cyclin D1−/− MEFs and cyclin D1−/− MEFs transduced with control vector (GFP) or human cyclin D1 (hD1wt). Nuclei were stained with 4′,6′-diamidino-2-phenylindole (DAPI). Scale bar, 50 μm. (D) Immunofluorescence analysis of cyclin D1+/+ or cyclin D1−/− MEFs and cyclin D1−/− MEFs transduced with vectors encoding human cyclin D1 (hD1wt) or vector control with antibodies for pY118-paxillin (red) and paxillin (green). Yellow dots indicate their colocalization. Typical examples of representative cells are shown. Note the centripetal distribution of tyrosine-phosphorylated paxillin in cyclin D1-deficient cells. Scale bar, 50 μm.