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. 2006 Jun;26(11):4161–4171. doi: 10.1128/MCB.02142-05

FIG. 5.

FIG. 5.

(A) ChIP assays showing the recruitment of the indicated proteins as well as K9 methylation of histone H3 on cdc25A and TS promoters upon induction of senescence. The binding of E2F1 was reduced in senescent cells. (B) A ChIP assay showing the recruitment of HDAC1, Brg1, and Brm on cdc25A and TS promoters in asynchronous, quiescent, or senescent cells. (C) A ChIP assay as described for panel B, showing the recruitment of the indicated proteins in serum-starved, quiescent MCF-7 cells. IP, immunoprecipitation. (D) PCR for cdc25A and TS promoters using different amounts of input DNA showing the linear range of the signal. (E) Real-time PCR showing repression of cdc25A and TS in senescent and quiescent cells. TS and Cdc25a were normalized to the average β-actin values for each cDNA sample. Error bars indicate standard deviations.