TABLE 2.

Distribution of macroH2A1 on active and inactive genes^a

Gene	Male	Female		Gene	Male	Female
Active genes
Albumin 1 (Alb1), chromosome 5				TATA box binding protein (Tbp), chromosome 17
1,800 bp before start	3.8 ± 0.69	4.0 ± 0.31
570 bp before start	1.2 ± 0.42	1.6 ± 0.11		Second exon	0.1 ± 0.01	0.2 ± 0.01
70 bp before start	0.8 ± 0.09	0.8 ± 0.22		Fifth exon	0.2 ± 0.01	0.2 ± 0.01
First intron	0.4 ± 0.07	0.4 ± 0.02
Twelfth intron	0.6 ± 0.08	0.7 ± 0.11		Transferrin (Trf), chromosome 9
2,110 bp beyond end	0.6 ± 0.06	0.7 ± 0.05		1,500 bp before start	2.8 ± 0.47	3.5 ± 0.33
				290 bp before start	0.2 ± 0.05	0.3 ± 0.04
ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 (Atp5c1), chromosome 2				First intron	0.5 ± 0.05	0.5 ± 0.04
	Sixteenth exon	0.3 ± 0.01	0.3 ± 0.01
	1,240 bp beyond end	0.5 ± 0.03	0.6 ± 0.02
First intron	0.2 ± 0.01	0.2 ± 0.01
Third intron	0.1 ± 0.02	0.2 ± 0.01		Ubiquinol-cytochrome c reductase hinge protein (Uqcrh), chromosome 4
Citrate synthase (Cs), chromosome 10					First intron	0.1 ± 0.01	0.1 ± 0.01
First intron	0.2 ± 0.03	0.3 ± 0.01		Second intron	0.1 ± 0.02	0.2 ± 0.01
Third intron	0.2 ± 0.06	0.2 ± 0.01
Cytochrome c, somatic (Cycs), chromosome 6				Potentially active genes
First intron	0.2 ± 0.01	0.1 ± 0.01		Alpha fetoprotein (Afp), chromosome 5
				580 bp before start	1.5 ± 0.18	2.0 ± 0.12
Enoyl coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), chromosome 7				Junction of third exon	0.6 ± 0.16	0.7 ± 0.1
	Twelfth intron	0.5 ± 0.08	0.5 ± 0.05
First intron	0.2 ± 0.02	0.2 ± 0.04
Fourth exon	0.3 ± 0.01	0.3 ± 0.01
				Inactive genes
Fibrinogen gamma (Fgg), chromosome 3				Myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), chromosome 14
2,115 bp before start	1.6 ± 0.11	2.2 ± 0.09
620 bp before start	0.5 ± 0.03	0.7 ± 0.09		1,750 bp before start	2.2 ± 0.25	2.0 ± 0.09
Third exon	0.3 ± 0.04	0.4 ± 0.05		Third intron	1.5 ± 0.21	1.5 ± 0.18
Seventh intron	0.3 ± 0.11	0.4 ± 0.09		Fourteenth intron	4.1 ± 0.12	4.5 ± 1.03
340 bp beyond end	0.4 ± 0.02	0.4 ± 0.04		Thirty-seventh intron	1.9 ± 0.1	1.7 ± 0.04
Glucokinase (Gck), chromosome 11				Glial fibrillary acidic protein (Gfap), chromosome 11
First intron	0.6 ± 0.05	0.8 ± 0.03
Second intron	1.1 ± 0.1	1.5 ± 0.2		First intron	1.6 ± 0.25	1.7 ± 0.04
Sixth intron	0.8 ± 0.01	1 ± 0.1		Seventh intron	2.9 ± 0.32	2.8 ± 0.37
Last exon	0.6 ± 0.01	0.8 ± 0.01
				Insulin I (Ins1), chromosome 19
Liver glycogen phosphorylase (Pyg1), chromosome 12				3′ exonb	0.7 ± 0.06	0.8 ± 0.06
	1,240 bp before start	0.9 ± 0.1	1.5 ± 0.08		Keratin (Krt2-19), chromosome 15
First intron	0.4 ± 0.03	0.4 ± 0.03		First intron	1.6 ± 0.17	1.8 ± 0.12
Next to second exon	0.5 ± 0.02	0.7 ± 0.03		Eighth intron, 1	1.7 ± 0.12	1.8 ± 0.16
				Eighth intron, 2	1.3 ± 0.07	1.2 ± 0.13
Spot 14 (Thrsp), chromosome 7
5′ exon	0.5 ± 0.06	0.6 ± 0.06		Trypsinogen (Try4), chromosome 6
Intron	0.5 ± 0.07	0.6 ± 0.1		First intron	0.5 ± 0.11	0.4 ± 0.07
3′ exon	0.3 ± 0.03	0.3 ± 0.03		Second intron	0.5 ± 0.1	0.5 ± 0.03
845 bp beyond end	0.3 ± 0.03	0.3 ± 0.04

^aValues are the ratios of the concentrations in the macroH2A1-enriched thiopropyl Sepharose eluted fractions to the concentrations in the starting materials, determined by real-time PCR.

^bThis probe may also detect insulin II.