TABLE 4.
Distribution of macroH2A1 on subtraction clones
| Clone(s) | Malea | Femalea | Repeat(s) presentb | Chromosome(s) | Nearest gene |
|---|---|---|---|---|---|
| Forward subtraction clones | |||||
| 1 | 2.7 ± 1.89 | 5.4 ± 0.65 | MaLR LTR and LINE L1 | 1 | Trpm8, intron |
| 5 | 2.1 ± 0.34 | 2.1 ± 0.2 | Abuts an MaLR LTR | 13 | LOC432934, >100 kb |
| 8 | 4.1 ± 0.49 | 4.1 ± 0.24 | GT repeat | 4 | Bach2, 15 kb |
| 13 | 5.8 ± 0.56 | 6.6 ± 0.84 | MIR | 6 | Atp2b2, intron |
| 24 | 6.0 ± 0.66 | 7.7 ± 0.83 | GT repeat and MIR | 9 | Edg8, 8.3 kb |
| 26 | 5.2 ± 0.6 | 5.3 ± 0.17 | Flanked by MaLR and ERVL LTRs | 11 | 1700019I23Rik, 27 kb |
| 27 | 5.0 ± 1.04 | 5.6 ± 0.3 | MaLR LTR | 2 | Gm356, 14 kb |
| 28 | 3.8 ± 0.36 | 4.2 ± 0.7 | RLTR4-int LTR | Multiple | Reverse transcriptase |
| 15, 18, 21 | 7.4 ± 0.24c | 9.3 ± 0.56c | 31-bp tandem repeat | X, Y, 9, 13 | Mid1 and others |
| 29 | 3.1 ± 0.16 | 3.2 ± 0.01 | MER1 and MIR | 6 | LOC269739, 19 kb |
| 32 | 3.1 ± 0.17 | 3.5 ± 0.28 | MaLR LTR and LINE L1 | 1 | Cnih3, intron |
| Reverse subtraction clones | |||||
| O5 | 0.2 ± 0.02 | 0.8 ± 0.57 | Repeated on Y and X | Y and X | |
| O7 | 0.5 ± 0.11 | 0.7 ± 0.09 | LINE L1 | Multiple | |
| H2 | 0.6 ± 0.07 | 0.7 ± 0.05 | LINE L1 | Multiple |
a
Values are ratios of the concentrations in the macroH2A1-enriched thiopropyl Sepharose eluted fractions to the concentrations in the starting materials, determined by real-time PCR. All target sequences were nonrepetitive except for clone 28 and the reverse subtraction clones.
b
Repeats were identified by Repeat Masker (50).
c
Result for a region 900 bp downstream of Mid1 between two clusters of these repeats.