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Bright, TFII-I, and Btk coprecipitate from the human B-cell line CL01. Whole-cell extracts from PMA-stimulated CL01 cells were immunoprecipitated (IP) with goat anti-Btk (C20), anti-TFII-I, anti-Bright, or control goat Ig (Anti-GtIg) and then immunoblotted (IB) for the presence of the indicated proteins. The first lane (load) contains 5% of the total protein used for each precipitation reaction. Relative molecular weights are indicated.
