FIG. 3.

Bright and TFII-I associate in a DNA-bound complex on the V1 promoter in a B-cell line. Antibody-facilitated chromatin immunoprecipitation was performed with stimulated BCg3R-1d B cells or the T-cell hybridoma KD3B5.8, using anti-Bright, anti-TFII-I, or goat Ig (Anti-GtIg). Immunoprecipitated DNA was PCR amplified at final dilutions of 1:100, 1:500, and 1:1,000 (represented by triangles) for the presence of the IgH V1 promoter. Ten percent of the DNA used for each immunoprecipitation was used as a positive control (Input).