FIG. 8.

Dominant negative TFII-I reduces immunoglobulin mRNA levels in a B-cell line. Serial dilutions of cDNA prepared from wild-type (WT), p70, or YYFF TFII-I transfected BCg3R-1d cells were PCR amplified for the V1 μ heavy chain and GAPDH. The results of two individual experiments are shown (a and b). Quantification of the V1 levels relative to the GAPDH level in each lane indicated threefold reductions relative to WT levels for p70 transcripts in both experiments and threefold and twofold reductions for the YYFF mutant in panels a and b, respectively. A Western blot indicates relative transfected protein levels in the lower portion of panel a. The positive control in the first lane (+) is an extract from Cos-7 transfected cells containing wild-type TFII-I.