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Recombinant TFII-I enhances Bright DNA-binding activity. Mobility shift assay mixtures containing extracts from Bright (Br)-expressing Cos-7 cells (neat in lane 2 or at a suboptimal 1:16 dilution in lanes 4 to 7) were incubated with 2, 3, and 4 μg (lanes 5, 6, and 7, respectively) of purified TFII-I prior to addition of the bf150 Bright binding site probe. Probe alone was assayed in lane 1. An arrow indicates the Bright complex.
