FIG. 3.
LPP is required for PEA3 target gene activity. (A to C) Reverse transcription-PCR analysis of endogenous MMP-1 expression. (A) 293 cells were transfected with the indicated combinations of expression vectors for PEA3 (400 ng), MEK(ΔN-S218E/S222D) (200 ng) and LPP (400 ng). (B) 293 cells were transfected with expression vectors for PEA3 (400 ng), MEK(ΔN-S218E/S222D) (200 ng) and the indicated LPP or control siRNAs. (C) MDA-MB-231 cells were transfected with the indicated LPP or control siRNAs.