FIG. 5.

LPP is required for PEA3-mediated promoter activation. Luciferase reporter gene assays were carried out in the presence of the indicated LPP or control siRNAs with the PEA3 site-driven (600 ng; A, B, and D) or Cox-2 promoter-driven (600 ng; C) luciferase reporter constructs. 293 cells were transfected with PEA3 expression constructs (600 ng) in the absence (A and C) and presence (B) of PMA stimulation. (D) MDA-MB-231 cells were transfected with reporter alone. Western blots showing the expression levels of LPP, PEA3, and GAPDH in the presence and absence of the indicated RNAi constructs are shown below the graphs.