FIG. 8.
Slm proteins are downstream of phytosphingosine. Saturated cultures of wild-type (SEY6210), slm1Δ (AAY1602), slm2Δ (AAY1610), and slm1ts slm2Δ (AAY1623.2) cells were serially diluted and spotted onto YPD, YPD plus 20 μM phytosphingosine (PHS), YPD plus 0.4 μg/ml myriocin, or YPD plus 0.4 μg/ml myriocin plus 20 μM phytosphingosine and grown for 2.5 days at the indicated temperatures. All plates contained 0.05% NP-40, which was used for obtaining even distributions of phytosphingosine in the plates.