FIG. 1.
Deletion of β-catenin in tamoxifen-treated β-cateninfl/fl/Cre mice. Cardiomyocytes were isolated from hearts of β-cateninfl/fl/Cre mice that had been treated with tamoxifen or vehicle (−Tamoxifen) for 5 days and then were sacrificed 10 days (top panels) or 6 weeks (A, bottom panel, and B) later. Myocytes were immunostained (A) or immunoblotted (B) for expression of β-catenin. (A, top left panel) The arrow identifies residual β-catenin remaining in the adherens junction. (A, bottom panel) The arrows identify cardiomyocytes in which β-catenin has been deleted by Cre-mediated recombination. (B) GAPDH serves as a loading control.