FIG. 7.

Effects of expression of various Bcl2 loop deletion mutants on its survival function. (A) Schematic representation of phosphorylation sites in the Bcl2 flexible loop domain. (B) WT and various loop deletion Bcl2 mutant cDNAs were stably transfected into H7 cells. Bcl2 protein expression levels were determined by Western blotting using a Bcl2 antibody. (C) Cells expressing WT or various loop deletion Bcl2 mutants were treated with cisplatin (20 μM) for 48 h. Cell viability was determined by analyzing annexin-V binding on a fluorescence-activated cell sorter. Data represent the mean ± standard deviation of three determinations.