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. 2006 Jun;26(12):4652–4663. doi: 10.1128/MCB.02193-05

FIG. 2.

FIG. 2.

PIASy promotes SUMO-2 conjugation to MafG through the lysine 14 residue. (A and B) The effect of PIAS family proteins on the sumoylation of His-MafG. His-MafG was transiently expressed in 293T cells, and sumoylation was examined by immunoblot analysis with anti-His antibody. SUMO-1 and SUMO-2 were expressed in panels A and B, respectively. (C and D) His-MafG K14R and His-MafG E16A were transiently expressed in 293T cells, and their sumoylation was examined in the absence or presence of PIASy. White and black arrowheads indicate MafG with or without sumoylation, respectively (A to D).