FIG. 6.

MafG-mediated transcriptional repression is sensitive to TSA treatment. (A) The effect of TSA treatment on MafG-mediated transcriptional repression. His-MafG, His-MafG K14R, or His-MafG E16A expression vector was transiently introduced into 293T cells with pRBGP2, p45, and His-MafG expression vectors. The relative luciferase activities with (right panel) or without (left panel) TSA treatment are shown. The relative amounts of the transfected plasmids are indicated. Error bars indicate the standard deviations for samples analyzed in triplicate. These results are representative of three independent experiments. (B) Relative derepression caused by TSA treatment. The derepression ratios of MafG and its mutants were calculated, from the result are shown in panel A.