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. 2006 Jun;26(12):4652–4663. doi: 10.1128/MCB.02193-05

FIG. 7.

FIG. 7.

Generation of G1HRD-His-MafG and G1HRD-His-MafG K14R transgenic mouse lines. (A) The mating strategy for the transgenic complementation rescue experiment. (B and C) Immunoblot analyses of whole-cell lysates from the bone marrows of transgenic mice carrying G1HRD-His MafG (B) or G1HRD-His-MafG K14R (C) using anti-small Maf antibody. Three independent lines (lines 8, 11, and 25) and four independent lines (lines 11, 18, 45, and 71) were established for G1HRD-His-MafG and G1HRD-His-MafG K14R transgenic mice, respectively. The band intensities of the transgene products relative to that of endogenous MafG are shown at the bottom. (D) Immunoblot analysis of bone marrow lysates from the compound mutant mice. Samples were prepared from G1HRD-His-MafG (lanes 3 and 4) or G1HRD-His-MafG K14R (lanes 5 and 6) mice, both in a mafG-null background, or wild-type (lane 1) or mafG-null (lane 2) mice. White and black arrowheads indicate MafG with or without sumoylation, respectively.