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. 2006 Jun;26(12):4474–4488. doi: 10.1128/MCB.01926-05

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FIG. 2. — Induction of Bim by TGF-β in SNU16 cells depends on RUNX3 function. (A) Expression profile of proapoptotic and antiapoptotic genes in SNU16 control clones (control), SNU16 clones expressing RUNX3(1-187) [RUNX3(1-187)], and SNU16 clones expressing exogenous RUNX3 in addition to endogenous RUNX3 (RUNX3) revealed by RT-PCR. (B) Expression of Bim in SNU719 control clones (control) and SNU719 clones expressing antisense DNA against RUNX3 (RUNX3-AS) revealed by RT-PCR. (C) Expression of Bim in the absence or presence of TGF-β in control, RUNX3(1-187), and RUNX3 revealed by Western blotting. (D) Comparison of Bim mRNA induction by TGF-β between control and RUNX3(1-187) by quantitative RT-PCR using two primer sets for detection of all splicing variants of Bim or BimL+BimEL.