FIG. 2.
Suppression of different phenotypes of THO mutants by multicopy THO1. (A) Northern analyses of tho2Δ (RK2-6C), mft1Δ (WMK-2A) cells carrying the Ptet::lacZ-URA3 construct, and thp2Δ (BY-HR167) carrying the GAL1pr::lacZ construct under transcription activation conditions. (B) Recombination frequency of the intrachromosomal direct-repeat system leu2-k::URA3-ADE2::leu2-k mft1Δ (MFM67-13A) and tho2Δ (WRK-1C) mutants transformed with multicopy vector YEp351 and YEp351-THO1. Details are as described for Fig. 1. (C) Suppression of the thermosensitivity (ts) phenotype of hpr1Δ, tho2Δ, mft1Δ and thp2Δ by multicopy THO1. Viability of hpr1Δ (U678-4C) tho2Δ (WRK-1C), mft1Δ (MFM67-13A), and thp2Δ (BY-HR167) cells transformed with either YEp351 or YEp351-THO1. As controls, we used wild-type (WT) W303-1A and BY4741. As they gave identical results, only one is shown. Transformants were spotted as 10-fold serial dilutions on selective medium. Photographs were taken after 3 or 5 days at 30 or 37°C, respectively, as indicated.