FIG. 6.

Effect of multicopy THO1 on Sub2 recruitment to active chromatin at the PMA1 gene in hpr1Δ and wild-type strains. ChIP analyses were performed with strains BSU-S2T.3B (WT) and BSU-S2T.6D (hpr1Δ) transformed with plasmid YEp351-THO1, carrying THO1 in multicopy or the empty vector. Other details are as described for Fig. 3. Error bars indicate standard deviations. The PCR fragment corresponding to the bp 9716 to 9863 intergenic region of chromosome V used as a control is indicated by asterisks.