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. 2006 Jun;26(12):4399–4409. doi: 10.1128/MCB.01147-05

FIG. 1.

FIG. 1.

Expression of Src constructs in SFK-null fibroblasts (SYF cells). (A) Representations of cSrc and mutants stably expressed in SYF cells. (B and C) Western blots indicating expression levels of exogenous proteins in SYF cells infected with myc-tagged Src251 (251) and Src251R175L (251RL) (B), as well as cSrc, SrcR175L, SrcY527F, and SrcR175L-FAT (SrcRL-FAT) (C). (D) Western blots of FAK phosphotyrosine levels in SFK-null fibroblasts (SYF) reconstituted with full-length (cSrc) and truncated constructs Src251 and Src251R175L (251RL). Cells were kept in suspension for 1 h (S), and levels of FAK pY397, in whole-cell lysates (WCL), and pY576, in FAK immunoprecipitates (IP), were compared to those for cells spread on fibronectin (F). The membrane was reprobed with anti-FAK MAb.