Localization of Src constructs reexpressed in SYF cells. The figure shows the cellular localization of chicken Src constructs stably expressed in SYF cells recognized by chicken-specific anti-Src antibody in fully spread cells on fibronectin. FAs were revealed by counterstaining with a FAK pY397-specific PAb. Src251 (A) (251), SrcR175L-FAT (B) (SrcRL-FAT), and SrcY527F (C) (Y527F) localized to FAs upon adhesion, whereas Src251R175L (D) (251RL), cSrc (E), and SrcR175L (F) (SrcRL) did not localize to FAs and were stained diffusely at the cell membrane.