FIG. 2.

(A) Human T98G glioblastoma cells were serum starved for 72 h, restimulated with 10% FBS with or without antioxidants, and harvested at 20 h (top panel). G₂/M human leukemic T cells (Jurkats) were synchronized by centrifugal elutriation, replated, and either not treated or treated with antioxidants. Zero and 16-h ROS levels in Fig. 1B correspond to DNA in the bottom panel. Cells were analyzed for DNA content by propidium iodide and FACS analysis. (B) T98G cells serum starved and restimulated with 10% FBS with or without antioxidants were pulsed with BrdU for 4 h to detect DNA replication and examined by immunofluorescence. (C) T98G cells treated as above and analyzed for cell size (forward scatter) and BrdU incorporation by FACS analysis.