FIG. 2.

hUbc9 does not restore cellular resistance to replication stress or CPT. ubc9-10 cells, transformed with YEp vectors expressing yUBC9 from its own promoter (yUBC9) or UBC9 alleles from the GAL1 promoter, were grown at 26°C, serially diluted, and spotted onto selective dextrose (Dex) or galactose (Gal) medium with or without HU (A) or onto selective Dex or Gal plates supplemented with HEPES, dimethyl sulfoxide, and CPT as indicated (B). Cell viability was assessed following incubation at 36°C.