FIG. 5.

Distinct yUbc9 domains enhance hUbc9 function in yeast ubc9Δ cells. Yeast-human (y/h) Ubc9 chimeras in GAL1-promoted vectors were introduced into ubc9Δ cells by plasmid shuffle (see Fig. 1B). The E1/Smt3 binding domain (1) and numbers referring to the first residue of the swapped domain are indicated in the yUbc9 scheme. yUbc9 sequences are in gray; hUbc9 sequences are in black. Photographs of independent strains, cured of YCpyUBC9 · U, were taken to emphasize differences in growth rates at 30°C.