FIG. 6.

y/hUbc9 chimeras fail to complement the defect in global SUMOylation. (A) As in Fig. 2, ubc9-10 top1Δ cells, transformed with YEp vectors containing yUBC9- or GAL1-promoted UBC9 alleles, were spotted onto selective dextrose (Dex) or galactose (Gal) medium with or without HU and incubated at 36°C. (B) As in Fig. 3, galactose-induced cell extracts (26°C or 36°C) were probed with anti-Ubc9 (α Ubc9) antibodies or antibodies specific for yUbc9 or Smt3. Staining of the nonspecific band (*) in the α Ubc9 panel mirrored tubulin controls.