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. 2006 Jul;26(13):4958–4969. doi: 10.1128/MCB.00160-06

FIG. 8.

FIG. 8.

Yeast Ubc9 N-terminal residues 22 to 38 alter cellular resistance to HU and MMS. (A) ubc9-10 top1Δ cells transformed with reciprocal N-terminal y/hUbc9 chimeras, in YCpGAL1 vectors, were spotted onto selective galactose medium plus HU or MMS and incubated at 26°C or 36°C. (B) Overlay of ribbon diagrams of yeast (green) and human (magenta) Ubc9, rotated 180° around the y axis from the view in Fig. 4B, and close-up solid bond representations of residues M1(S3) to F22 and F22 to L38 to emphasis differences in side chains. (C and D) Immunoblots of ubc9-10 top1Δ cells expressing the indicated UBC9 allele at 36°C, stained with anti-Ubc9 (α Ubc9) (C) or α Smt3 (D) antibodies. *, nonspecific bands mirrored tubulin controls.