Figure 4.

HPLC separation of lipophilic compounds from embryogenic cultures of Norway spruce. Suspension cultures (20 mL) of cell line B1 were grown in the presence of N-acetyl-d-[1-¹⁴C] glucosamine for 14 d. A larger volume (2 L) of this cell line was also cultured without radiolabeled precursor. Lipophilic compounds were extracted by reverse-phase cartridge and separated by reverse-phase HPLC. A, HPLC fractions of radiolabeled extract assayed for ¹⁴C radioactivity. B, Amount of GlcNAc measured in extract before separation (non-sep), and in aliquot of fractions with Rts: 0 to 1, 2 to 4, 5 to 6, 7 to 12, 13 to 17, 18 to 22, 23 to 26, 27 to 29, and 30 to 36 min.