Table I.
Effect of GlcN-positive lipophilic compounds on PEM proliferation
| Variant | GlcN | PEMs |
|---|---|---|
| ×10−8m | % | |
| Control | 8.5 | |
| 2,4-D | 17.6a | |
| 80% (v/v) methanol extract, nonseparated | 2.0 | 14.7a |
| Fraction A | 0.5 | 13.6a |
| Fraction B | 0.2 | 10.8 |
| Fraction C | 0.8 | 15.9a |
| Fraction D | <0.1 | 7.5 |
Lipophilic extracts from culture filtrates of cell line B1 were separated by HPLC. The content of GlcN was measured by GC-MS in the nonseparated 80% (v/v) methanol extract and in fractions with retention times (min): fraction A, 0 to 4; fraction B, 5 to 9; fraction C, 13 to 17; fraction D, 18 to 26. Equivalent aliquots of the nonseparated extract or fractions A, B, C, and D were added to the 80- to 160-μm fraction of A22 cultures. As an alternative, 9 × 10−6 m 2,4-D was added to the fractionated cultures. The frequency of proliferating PEMs was determined after 3 weeks. The data are based on 2,000 PEMs per trial.
Significantly different from the control as estimated using Z-test (P ≤ 0.05).