Table II.
Effect of chitinase treatment on lipophilic compounds from embryogenic cell line B1 of Norway spruce
| Variant | Pretreatment with Chitinase | GlcN | PEMs |
|---|---|---|---|
| ×10−8m | % | ||
| Control | 8.00 | ||
| 2,4-D | 18.20a | ||
| Nod factor NGR234 | 14.10a | ||
| 80% (v/v) methanol extract, nonseparated | − | 1.6 | 17.50a |
| Fraction C | − | 0.7 | 16.15a |
| Fraction C | + | <0.1 | 6.90 |
Equivalent aliquots of the nonseparated 80% (v/v) methanol extract were subjected to incubation without (−) or with (+) chitinase from S. griseus and thereafter separated by HPLC. Radiolabeled compounds were used as markers for HPLC separation. The GlcN content of the fractions coeluting with 14C radioactivity was measured by GC-MS. Equivalent aliquots of the nonseparated extract, fraction C of the non-digested extract, or fraction C of the extract treated with chitinase were added to the 80- to 160-μm fraction of A22 cultures. As an alternative, 9 × 10−6 m 2,4-D or 10−8 m Nod factor NGR234 was added to the fractionated cell cultures. The frequency of proliferating PEMs was determined after 3 weeks. The data are based on 2,000 PEMs per trial.
Significantly different from the control as estimated using Z-test (P ≤ 0.05).