FIG. 7.

Endogenous MondoA regulates glycolysis. (A) Real-time PCR measured enrichment of ChIPs from BJ fibroblasts performed with MondoA, c-Myc, or Gal4p antibodies. Immunoprecipitated DNA was analyzed by real-time PCR with primers specific to the E-box-containing regions of the human LDH-A, HKII, and PFKFB3 promoters. Measurements are expressed as fold enrichment over the amount of an upstream region of the PFKFB3 promoter that does not contain a CACGTG site. Each value is the average (±the standard error) from at least three independent biological replicates. Expression levels of MondoA and tubulin (B) and glycolysis rates (C) in K562 cells expressing the indicated shRNA constructs are also shown. NS, a scrambled shRNA that does not recognize any sequence in the human genome; M1 and M2, shRNA sequences specific for human MondoA.