FIG. 5.

Overexpression of the stress-responsive transcription factor MSN4 extends life span and decreases ROS in GS129. (A) Flow cytometric analysis of stationary-phase wild type (WT) and GS129 containing either empty vector or a high-copy plasmid containing MSN4. Samples were taken at day 2 of stationary phase and are reported as described in the legend to Fig. 3. (B) Viability (at day 5 of stationary phase) of the strains described for panel A, determined by plating 10-fold serial dilutions onto rich medium (YPD).