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. 2006 Jul;26(13):4843–4852. doi: 10.1128/MCB.02267-05

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Copyright © 2006, American Society for Microbiology

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FIG. 2. — Chromatin binding of RecQ4 in interphase extracts of Xenopus eggs. (A) Specificity of anti-RecQ4 antibody. Interphase egg extract was resolved by SDS-PAGE and immunoblotted with anti-RecQ4 antibody. (B) Time course of chromatin binding of RecQ4 and other replication proteins under various conditions. Xenopus sperm chromatin was incubated in egg extracts at 23°C in the absence (Control) or the presence of 15 μg/ml green fluorescent protein-geminin (+geminin), 50 μg/ml GST-p21 plus 200 μM roscovitine (+p21/ros.), or 40 μg/ml aphidicolin (+Aphi) for the indicated times. The chromatin fractions isolated by centrifugation and the egg extract (1 μl) were resolved by SDS-PAGE and immunoblotted with antibodies indicated in the figure. Negative control of chromatin fractions was obtained by incubating the extracts in the absence of sperm chromatin (−sperm).