FIG. 3.

Synthesis and steady-state levels of OXPHOS proteins in COX10 KO cells. (A) We analyzed the steady-state levels of several subunits of cytochrome oxidase by Western blotting, including Cox1, Cox4, Cox5b, Cox6b, and cytochrome c. The lower part of the panel shows the steady-state levels of subunits of complex I (Ndufa9), complex II [SDH(Fp)], complex III (Uqcrc2 and Uqcrfs1), and complex V (ATPase-β). Protein loading was analyzed using an antibody against VDAC1. (B) [³⁵S]methionine-labeled mitochondrial proteins (60 μg) were separated on SDS-15% PAGE. Fluorographic bands were assigned as described previously (11). LMTK ρ°, mouse cell line devoid of mtDNA. (C) Southern analyses of total DNA digested with SacI or with NheI. The membrane was hybridized with a ³²P-labeled mitochondrial probe (mouse mtDNA nucleotide positions 5556 to 6268). Ctrl, control; cyt, cytochrome.