FIG. 5.

Northern blot analysis of human FAT10 mRNA. A. Northern blot of multiple tissues from healthy humans. Lane 1, spleen; 2, thymus; 3, prostate; 4, testis; 5, ovary; 6, small intestine; 7, colon mucosal lining; 8, peripheral blood leukocytes. Lane M contains molecular size markers. B. Analysis of total RNA blots from various cell lines. The origins of cell lines are indicated above the gel, and the two rRNA bands are indicated as 28S and 18S to the left of the gel. C. IFN-γ treatment for 0, 18, and 36 h induces progressive increases in FAT10 mRNA expression in HeLa and JY human cell lines.