FIG. 1.
Generation of conditional EnppF/+ mice. (A) Schematic representations of the Enpp2 locus, the targeting construct, and the mutated Enpp2 alleles. Exons are indicated by filled boxes, LoxP sites by filled triangles, and FRT sites by open triangles. Probes P1, P2, and P3 are indicated. H, HindIII sites; P, PstI sites; A, ApaI sites. (B) Identification of correctly targeted ES cell clones. Genomic DNA was digested with ApaI and screened with a 3′ flanking probe (P1) located outside the targeting construct. (C and D) Genotyping of adult mice and E9.5 embryos with conditional Enpp2F and/or deleted Enpp2Δ alleles by use of PCR with primers 1F, 1R, and 2R or PstI-digested DNA screened with P3.