Figure 2.

Southern-blot analysis of SIRK gene copy number. A, Blot of total genomic DNA (10 μg per lane) digested with the indicated restriction enzymes. Hybridization was performed with the 3.2-kb PflmI/DraI fragment of SIRK cDNA. The blot was washed under high-stringency conditions. The positions of the size standards are indicated on the left in kilobases. B, Restriction map of SIRK gene. The localization of restriction enzyme sites (EI, EcoRI; EV, EcoRV; and H, HindIII) and the position of the probe are indicated in bases from the translational start codon of the gene.