Figure 1.

Selection for differentiation-defective trypanosome lines. (a) VSG coat loss for trypanosome lines subjected to zero (Round 0; T. b. rhodesiense EATRO 2340 GUP2965.3; the starting wild-type population), three, four, and five rounds of in vitro differentiation and in vivo selection. (b) Expression of the procyclic stage-specific coat procyclin during the selection regimen. (c) VSG coat loss for DiD-1, a clonal line derived from Round 4 of the selection regimen, shown in comparison with the wild-type population. (d) Differentiation of the DiD-1 population assessed by gain of procyclin.