Figure 4.
DiD-1 does not show a cell cycle defect. (a) BrdU incorporation of DiD-1 and wild-type cells in culture at 27°C, with 6 mM cis-aconitate. In the left graph, cells were incubated in the presence of 50 mM BrdU and 50 mM 2′-deoxycytidine from the outset. In the right graph, BrdU and 2′-deoxycytidine were not added until 8 h after the cells had been placed into differentiation conditions. (b) Cell cycle progression of wild-type and DiD-1 cells during the course of a bloodstream parasitemia. The proportions of cells with a configuration of one kinetoplast and one nucleus (1K1N), 2K1N, and 2K2N were scored for each population when the parasite density was 5 × 107 cells/ml (low) or 5 × 108 cells/ml (high). The data represent four individual parasitemias for each cell type.