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GPI-PLC gene deletion in DiD-1. (a) Genomic Southern blots of DiD-1 cells (DI), DiD-1 cells deleted for one allele of GPI-PLC (D13), or DiD-1 cells deleted for both alleles of GPI-PLC (D133 and D135). Each was digested with BamHI and probed with the gene for GPI-PLC. (b) Development of stumpy cell characteristics after prolonged growth of the DiD-1–3.5 GPI-PLC null mutant in the bloodstream. Bar, 10 μm.
