Figure 3.

Concentration-dependent effect of NPA on gravistimulated curvature and Ivr2 transcript accumulation in maize stem pulvini. Maize explants were gravistimulated with the indicated concentration of NPA. After 24 h, angle of gravitropic curvature was measured and whole pulvini were harvested. Total RNA was isolated from pulvini tissue and was hybridized with an Ivr2 cDNA probe, radioactivity stripped, and the membrane was rehybridized with 18S rRNA probe. Mean values for gravitropic curvature (±se) from three independent experiments and a representative northern from one experiment (inset) are shown.