Phylogenetic comparison of the E.
lagascae CYP726A1 with selected members of the cytochrome P450
superfamily. The unrooted phylogenetic tree shown was generated by use
of the neighbor-joining algorithm (Saitou and Nei, 1987). The P450s
represented (and corresponding GenBank accession numbers) are as
follows: CYP1A1, human (Homo sapiens) arachidonic acid
epoxygenase (K03191); CYP2E1, Mus musculus lauric acid ω-1
hydroxylase (X62595); CYP2J2, human arachidonic acid epoxygenase
(U37143); CYP94A2, common vetch (Vicia sativa) medium chain
fatty acid hydroxylase (AF092917); CYP94A1, common vetch fatty acid
ω-hydroxylase (AF030260); CYP86A1, Arabidopsis fatty acid
ω-hydroxylase (X90458); CYP4A1, Rattus norvegicus fatty
acid ω-hydroxylase (M57718); CYP74B1, Capsicum annuum
fatty acid hydroperoxide lyase (U51674); CYP74A1, flax
(Linum usitatissimum) allene oxide synthase (U00428);
CYP102, Bacillus megaterium fatty acid in-chain
hydroxylase (J04832); CYP73A1, Jerusalem artichoke (Helianthus
tuberosus) cinnamate 4-hydroxylase (Z17369); CYP79A1,
Sorghum bicolor Tyr N-hydroxylase (U32624);
CYP703A1, Petunia × hybrida lauric acid
monooxygenase (AB006790); CYP76B1, Jerusalem artichoke 7-ethoxycoumarin
O-deethylase (Y09920); CYP81B1, Jerusalem artichoke fatty
acid in-chain hydroxylase (AJ000477); CYP71A1, avocado (Persea
americana) monoterpene monooxygenase (M32885); CYP71D8,
soybean elicitor-induced P450 (unknown function) (O81974);
CYP71D13, Mentha sp. (-)-(4S)-limonene-3-hydroxylase
(AF124816); CYP71D4, potato fungus-induced P450 (unknown function)
(AJ296346); and CYP71D12, Catharanthus roseus tabersonine
16-hydroxylase (AJ238612); and CYP71D16, tobacco cembratriene-ol
hydroxylase (AF166332). The E. lagascae CYP726A1
(AF406732) is highlighted in gray.