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. 2002 Feb;128(2):615–624. doi: 10.1104/pp.010768

Figure 4.

Figure 4

GC analysis of fatty acid methyl esters prepared form yeast cells expressing the E. lagascae CYP726A1 cDNA in media supplemented with linoleic (B) or α-linolenic acid (D). Gas chromatograms in A and C show fatty acid methyl esters from yeast cells harboring the expression vector without cDNA insert and grown in the presence of exogenous linoleic and α-linolenic acids, respectively. The gas chromatogram in E corresponds to fatty acid methyl esters prepared from E. lagascae seeds. Based on mass spectral analyses shown in Figure 5, the peak labeled Epoxy1 in B is identified as the methyl ester of vernolic acid, and the peak labeled Epoxy2 in D is tentatively identified as the methyl ester of 12-epoxyoctadeca-9,15-dienoic acid. Other labeled peaks correspond to methyl esters of the following fatty acids: 16:0, palmitic acid; 16:1, palmitoleic acid; 18:0, stearic acid; 18:1, oleic acid; 18:2, linoleic acid; and 18:3, α-linolenic acid.