GC analysis of fatty acid methyl esters prepared
form yeast cells expressing the E. lagascae CYP726A1 cDNA in
media supplemented with linoleic (B) or α-linolenic acid (D). Gas
chromatograms in A and C show fatty acid methyl esters from yeast cells
harboring the expression vector without cDNA insert and grown in the
presence of exogenous linoleic and α-linolenic acids, respectively.
The gas chromatogram in E corresponds to fatty acid methyl esters
prepared from E. lagascae seeds. Based on mass spectral
analyses shown in Figure 5, the peak labeled Epoxy1 in B is identified
as the methyl ester of vernolic acid, and the peak labeled Epoxy2 in D
is tentatively identified as the methyl ester of
12-epoxyoctadeca-9,15-dienoic acid. Other labeled peaks correspond to
methyl esters of the following fatty acids: 16:0, palmitic acid; 16:1,
palmitoleic acid; 18:0, stearic acid; 18:1, oleic acid; 18:2, linoleic
acid; and 18:3, α-linolenic acid.