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. 2002 Feb;128(2):615–624. doi: 10.1104/pp.010768

Figure 6.

Figure 6

GC analysis of fatty acid methyl esters prepared from tobacco callus transformed with the expression vector lacking cDNA insert (A) or from tobacco callus expressing the E. lagascae CYP726A1 cDNA under control of the cauliflower mosaic virus 35S promoter (B). Shown in C is a gas chromatogram of fatty acid methyl esters prepared from E. lagascae seed. Based on mass spectral analyses, the peak in B labeled Epoxy1 corresponds to the methyl ester of vernolic acid, and the peak labeled Epoxy2 is tentatively identified as the methyl ester of 12-epoxyoctadeca-9,15-dienoic acid. Peaks labeled with asterisks correspond to phytol.